Below are tables showing the compatibility of common sequencing primers with common plasmids. Since we cannot provide information on all primers / plasmids in use, if you do not see the information you need, please check for compatibility yourself. We do have some advice on checking primer compatibility.

In the tables below, "+" indicates that the priming site is present. "-" indicates that, to the best of our knowledge, the priming site is not present.

Common Cloning Vectors

Vector	Mnfr	T7	T3	M13F	M13R	KS	SP6	T7 Term
pBluescript I & II	Stratagene	+	+	+	+	+	-	-
pPCR Script	Stratagene	+	+	-	+	+	-	-
pCR2.1 ,-XL, -Blunt	Invitrogen	+	-	+	+	-	-	-
pCR-II, pCR-Blunt-II	Invitrogen	+	-	+	+	-	+	-
pGEM-1, 9Zf	Promega	+	-	-	-	-	+(*)	-
pGEM-3Z; -3,5,7,11,13Zf	Promega	+	-	-	-	-	+	-
pGEM-4Z	Promega	+	-	+	+	-	+(*)	-
pGEM-T, -T Easy	Promega	+	-	+	+	-	+	-
pT7T3	Pharmacia	+	+	-	-	-	-	-
Lambda Zap	Stratagene	+	+	+	+	+	-	-

* There is a 4 bp mismatch at the 5' end of the priming sequence in this vector.

pGEX GST-fusion Vectors

Vector	Mnfr	pGEX-F	pGEX-R
pGEX-1, pGEX-1lT	Pharmacia	+	+
pGEX-2T, pGEX-2TK	Pharmacia	+	+
pGEX-3X	Pharmacia	+	+
pGEX-4T1, -4T2, -4T3	Pharmacia	+	+
pGEX-5X1, -5X2, -5X3	Pharmacia	+	+
pGEX-6P1, -6P2, -6P3	Pharmacia	+	+
pEBG-2T	N/A	+	?

T7 Promoter Vectors

Vector	Mnfr	T7	T7 Term
pET (all known)	Novagen	+	+
pT7-7	N/A	+	?

Baculovirus Expression Vectors

Vector	Mnfr	Forward	Reverse
pFastBac-1	Life Technologies	+	+
pFastBac-HTa, -HTb, -HTc	Life Technologies	+	+

Mammalian Expression Vectors

Vector	Mnfr	CMV-F	CMV-R	T7	SP6
pCDNA3	Invitrogen			+	+(*)
pCMV5		+	+

* The "T" residue at base 4 of the new SP6 primer is not present in the pCDNA3 SP6 priming site.