High quality plasmid DNA is essential to achieve the best results. We would recommend a good quality mini-prep kit (e.g. Promega Wizard SV or Qiagen) or maxi-prep DNA. Large templates are difficult to purify in either sufficient amount or purity to give very good DNA sequence. Please ensure that you follow any kit instructions carefully to obtain the best yield and purity of template. It is also very important with these templates to quantify them before sending to us. However, if you would like to save time, we are happy to take care of this step for you. Just click ‘Quanti-it’ on the sample submission page.
There are many factors that can affect the read length one obtains. Our facility will endeavour to provide customers with the longest read possible depending on service requested. Our new ABI 3730 sequencer uses the latest technology and provides really excellent results.
With a template of appropriate quality, customers can expect read lengths of up to 1000 bases of sequence from a single primer when sequencing standard templates (<20kb). For other types of template (especially for large templates), read lengths can decrease to issues of template quality and quantity. If you have problems achieving good read lengths with high quality template, please take a look at our resources section. If you still need more help, please feel free to contact us.