In most cases, we initiate sequencing from both ends of the insert and when the sequences overlap in the middle, we stop (or carry on and fully sequence both strands if this is what you have asked for). In some cases, we may only be able to sequence from one end (e.g. due to polyA tail on cDNA, or because of some other problem). If this happens, we will contact you right away.
The costs of all sequencing primers/reactions and assembly of the final sequence are included. You will receive back the completed sequence plus all primers synthesised for the project. Data acquisition is about 1kb a week (if sequencing from both ends) and we can also provide you with the sequence as it is generated.
This service is optimal for ‘walks’ of between 2-10 kb (not including plasmid backbone). If the region you wish to be sequenced and analysed lies outside this size, please contact us
MRC PPU DNA Sequencing and Services will sequence the entire insert – on one or both strands, as requested.
Analysis of ‘Walks’
Please note that we cannot guarantee to be able to complete primer walking projects, since secondary structures and difficult regions of sequence can make it challenging to read through certain sequences. However, we do try our best and if we cannot complete the project, we will just charge for the sequence that we can obtain.